Sunday, February 5, 2023
News

Researchers reveal how metastatic cancer causes leaky blood vessels

   SocialTwist Tell-a-Friend    Print this Page   COMMENT

Washington | December 7, 2022 12:32:09 PM IST
Researchers examine the local communication between endothelial cells and tumors cells and its effects on endothelial cell orientation. The approach uses co-cultured human umbilical vein endothelial cells and breast epithelial tumor cell lines to simulate the tumor-endothelial interaction. The group found the clockwise chirality of the hUVECs was less affected by local hormone signaling and more so by direct physical contact with tumor cells. Specific proteins on the tumor cell binding to others on endothelial cells appeared to play a role in changing the clockwise chirality of hUVECs.

In APL Bioengineering, by AIP Publishing, researchers from the University of Michigan-Dearborn, Rensselaer Polytechnic Institute, and the University at Albany -- SUNY developed a model that examines the local communication between endothelial cells and tumors cells and its effects on endothelial cell orientation. The approach involves the use of co-cultured human umbilical vein endothelial cells (hUVECs) and breast epithelial tumor cell lines to simulate the tumor-endothelial interaction.

"The blood vessels in tumor tissues are much leakier than those in normal tissues," said author Jie Fan. "We are curious whether the tumor cells can induce the weakening in the clockwise orientation of endothelial cells and cause them to disorganize in the vessel."

The work expands on recent findings that showed that endothelial cells have chirality, a type of mirrored-image orientation similar to right and left hands, and they tend to skew in a clockwise direction.

"We believe the strong clockwise chirality of endothelial cells is important to maintain blood vessel integrity, but unfortunately, it could be damaged/weakened by tumor cells, which may increase the risk of metastasis," Fan said. "Preserving the normal chirality could inhibit tumor transmigration by reinforcing the chirality and the integrity of the endothelial barrier of blood vessels."

To address the question, the group co-cultured hUVECs with a series of tumor cell lines with varying degrees of malignancy. They then compared how hUVECs responded when the tumor cells were directly touching them versus not. To exert such a degree of control, they employed a contact printing technique to create either donut- or figure eight-shaped micropatterns to hold the cells.

The group found the clockwise chirality of the hUVECs was less affected by local hormone signaling and more so by direct physical contact with tumor cells. Specific proteins on the tumor cell binding to others on endothelial cells appeared to play a role in changing the clockwise chirality of hUVECs.

The group was surprised by the movement of the cell types. In most models for metastasis, it is thought that tumor cells march toward blood vessels before breaking through into the bloodstream.

"We had expected the tumor cell would invade the endothelial cell," Fan said. "However, we found the endothelial cells were moving towards the tumor cell on the micropattern." (ANI)

 
  LATEST COMMENTS (0)
POST YOUR COMMENT
Comments Not Available
 
POST YOUR COMMENT
 
 
TRENDING TOPICS
 
 
CITY NEWS
MORE CITIES
 
 
 
MORE HEALTH NEWS
Obesity can lead to 13 different types o...
Cancer projections for 2021-25 show a wo...
Intermittent fasting might reverse type ...
Study reveals how breast MRI is effectiv...
Researchers find link between traffic no...
Gujarat girl gets a 'new hand' after 13-...
More...
 
INDIA WORLD ASIA
Nagaland polls: Drugs, cash valued at Rs...
Will work together with youth to make Ma...
Meditation helpful for anorexia patients...
Women protest against Assam govt's crack...
'No one can give warning...' Law Ministe...
Maharashtra: Aaditya Thackeray terms BMC...
More...    
 
 Top Stories
I am used to jumping into tournamen... 
22 killed in Chile wildfires, say o... 
Kit Harington, Rose Leslie of 'Game... 
TVS Racing's Aishwarya Pissay eyes ... 
Uttarakhand CM condoles ANI's Chief... 
Earthquake of 3.1 magnitude hits Te... 
Gymnast Dipa Karmakar admits to dop... 
Pashtuns subject to extrajudicial k...